Marcadores de DNA basados en la PCR: AFLPs | 23/95 | UPV

About this Video

• Video Title: Marcadores de DNA basados en la PCR: AFLPs | 23/95 | UPV
• Channel: Universitat Politècnica de València - UPV
• Speakers: The transcript does not name the speaker.
• Duration: 00:10:20

Introduction

This video explains DNA markers based on PCR, specifically AFLPs (Amplified Fragment Length Polymorphisms). The video aims to provide students with the foundational knowledge to describe the process of polymorphism detection using AFLP markers, detail the causes of polymorphism, and justify the advantages and disadvantages of this marker type.

Key Takeaways

• AFLP Procedure: The video details the AFLP process, including DNA extraction, digestion with restriction enzymes (one rare-cutting and one frequent-cutting), adapter ligation, and two PCR amplifications (pre-amplification and selective amplification).
• Polymorphism Sources: Polymorphisms in AFLPs result from changes affecting restriction enzyme sites (point mutations, insertions, or deletions), changes in the 3' region of the added bases to the adapter, and small insertions or deletions within amplified regions.
• AFLP Advantages: AFLPs offer high reproducibility, require no sequence information, generate large amounts of information per reaction, and don't need probe generation.
• AFLP Disadvantages: AFLPs are dominant markers (cannot differentiate homozygotes), can be technically complex, and have issues with fragment size ambiguity (same size fragments may originate from different loci).