About this video

• Video Title: Peter Magic Janoshik PepTok Interview
• Channel: PepTok
• Speakers: Rory, Peter Magic
• Duration: 00:48:58

Overview

This video is an interview with Peter Magic from Janoshik, a company that provides testing services for the peptide industry. The conversation covers the origin of the Janoshik name, how Peter accidentally started the business, the evolution of peptide testing, the types of tests offered, and advice on storage, usage, and the reliability of testing agencies.

Key takeaways

• Origin of Janoshik: The name Janoshik originates from a Slovakian folk hero, adopted by Peter Magic from online forums to distinguish himself and later stuck with his business.
• Accidental Beginnings: Peter Magic started Janoshik by testing performance-enhancing drugs for friends, which grew organically due to demand for reliable testing.
• Peptide Testing Growth: Peptide testing, particularly for GLP-1 agonists like semaglutide and tirzepatide, has exploded in recent years, now constituting 70-80% of Janoshik's business.
• Types of Tests: Janoshik offers mass spectrometry screening, endotoxin testing, sterility testing, and elemental analysis (CHNS) for peptides. Heavy metal testing is also available but considered less necessary by Peter.
• Test Reliability & Divergence: Differences in testing results between agencies can arise from variations in methodology, calibration, or procedural errors. Blind, group testing is emphasized as a way to ensure accountability.
• Storage and Shelf Life: Properly manufactured lyophilized peptides are very stable, with some stored for a decade at room temperature still showing effectiveness, though refrigeration or freezing is recommended for longer-term storage.
• Reconstituted Peptides: Reconstituted peptides are generally advised to be used within 2-4 weeks, with a month being a comfortable limit when stored properly to avoid microbial contamination or local reactions.
• Bacteriostatic Water Concerns: The quality and purity of bacteriostatic water can be a concern, with some generic brands showing zero benzyl alcohol or being unsterile. Using pharmaceutical-grade sterile water or saline for injection is suggested as a safer alternative.
• Failure Rates: Janoshik has a failure rate of approximately 5% for peptide samples that either contain significantly different dosages, the wrong peptide, or no peptide at all. Sterility testing failures are separate.

Using contaminated or degraded reconstituted peptides can lead to several consequences. Microbial contamination can cause local reactions at the injection site. While the peptide compound itself might still be present, its degradation can reduce its effectiveness. In cases of significant contamination, the presence of living organisms can lead to more severe issues and local reactions.

The generally advised usage period for a reconstituted peptide is typically four weeks when stored properly in a refrigerator. While some peptides might remain viable for longer (six to eight weeks), it's not recommended due to the increased risk of microbial growth and potential for local reactions. Using the smallest possible vial size that fits your needs is also suggested for safety.