This video explains the electron transport chain (ETC) as the fourth step of cellular respiration. It focuses on how complexes 1, 3, and 4 pump protons (H+) from the mitochondrial matrix to the intermembrane space, creating a proton gradient. This gradient drives ATP synthesis through a process involving ATP synthase, which acts as a channel and enzyme to produce ATP. The video also touches on the resulting pH differences and the principle of diffusion from high to low concentration.